Journal: Cell reports
Article Title: SIRT2 suppresses aging-associated cGAS activation and protects aged mice from severe COVID-19
doi: 10.1016/j.celrep.2025.115562
Figure Lengend Snippet: Young (5 months old) or old (24 months old) mice were inoculated intranasally with control or ORF3a-expressing AAV6. Mice were analyzed 3 weeks later. n =2 independent experiments. (A and B) Quantitative real-time PCR analyses of the mRNA levels of the indicated genes in BALF cells of young and old mice. n = 8. (C) Quantitative real-time PCR analyses of the mRNA levels of IFNβ in the lungs of young mice. n = 6. (D and E) H&E staining (D) and quantification (E) of lung sections of young mice. Scale bar: 100 μm n = 6 mice/group, 10–15 images examined from 3 slides/mouse. (F) Flow cytometry analysis of myeloid cells (Gr1 + CD11b + ) in BALF of young mice. n = 6. (G and H) Immunostaining for Ly6G + cells (G) and quantification (H) of lung sections. Scale bar: 100 μm n = 8 mice/group, 6 images examined from 3 slides/mouse. (I and J) Immunostaining for CD68 + cells (I) and quantification (J) of lung sections. Scale bar: 100 μm n = 8 mice/group, 4 images examined from 3 slides/mouse. (K and L) Sirius red staining (K) and quantification (L) of lung sections. Scale bar: 200 μm n = 6 mice/group, 10 images examined from 3 slides/mouse. (M and N) Immunostaining for phosphorylated STING (M) and quantification (N) of lung sections of young and old mice. Scale bar: 30 μm n = 8 mice/group, 5 images examined from 3 slides/mouse. Data are mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. See also – .
Article Snippet: CD68 clone FA-11 Rat monoclonal antibody , Bio-Rad , Cat# MCA1957GA; RRID:AB_324217.
Techniques: Control, Expressing, Real-time Polymerase Chain Reaction, Staining, Flow Cytometry, Immunostaining
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